ABSTRACT
BACKGROUND: The gold nanoparticles have a killing effect on tumor cells.OBJECTIVE: To investigate the influence of gold nanochain on Hep-2 cells proliferation of human laryngeal carcinoma.METHODS: The gold nanochain was prepared by a glucose synthesis method and added into the culture cells with different concentrations (10%, 25%, 50%, 75%, 95%) to test the influence on proliferation of in vitro cultured Hep-2 cells. The endocytosis and exocytosis of transmembrane when gold nanochain attached to Hep-2 cells were observed by electron microscopy.RESULTS AND CONCLUSION: The gold chain at high concentrations (75%, 95%) exhibited inhibitory effects on the proliferation of Hep-2 cells, but the influence was not increased with increasing concentration, belonging to a range of non-toxic. Gold nanchain can enter into Hep-2 cells after 8 hours of co-culture and leave cells after 48 hours, indicating gold nanoparticles chain can enter and leave Hep-2 freely.
ABSTRACT
OBJECTIVE@#To identify the newborns who should receive hearing evaluation by hearing screening in high risk newborns; to find and confirm the high risk factors of hearing disorders in high risk newborns.@*METHOD@#The first screening was performed by DPOAE. Newborns did not passed the first screening undertook second screening using DPOAE + ABR. and newborns did not passed the second screening received hearing evaluation. High risk factors of hearing loss were found by Logistic regression analysis.@*RESULT@#Three hundred and twenty-seven cases were screened. The positive ratio in first screening was 37.0%. The positive ratio in second screening was 11.0%. Ten cases were diagnosed as hearing loss and the incidence of hearing loss was 3.39%. High risk factors of hearing loss were asphyxiation, very low born weight (<1,500 g) and head and neck abnormality.@*CONCLUSION@#(1) DPOAE combined with ABR is credible and feasible in hearing screening of high risk newborns. (2) High risk factors of hearing loss were asphyxiation, very low born weight (<1,500 g) and head and neck abnormality in this study.
Subject(s)
Female , Humans , Infant, Newborn , Male , Hearing Disorders , Diagnosis , Epidemiology , Hearing Loss , Diagnosis , Epidemiology , Hearing Tests , Incidence , Neonatal Screening , Risk FactorsABSTRACT
OBJECTIVE@#This study aimed to analyze the expression of epidermal growth factor receptor (EGFR), Ki67 and p16 in human middle ear cholesteatomas and to investigate the correlation between its expression and the ability of erosion of cholesteatoma.@*METHOD@#The specimens from the acquired middle ear cholesteatoma tissue of 30 cases and 21 external ear canal skin samples from patients and 17 external ear canal skin samples from healthful men were taken intraoperatively. Their expression was examined by immunohistochemical SP method. Then we scanned it into a computer by an image scanner and quantified the gray of them using commercial software.@*RESULT@#The percent of positive expression of EGFR, Ki67 and p16 in middle ear cholesteatoma were 70.0%, 60.0%, 46.7%. Their expression tended to the increased greatly compared with the skins of the control groups. There was not correlation between the expression of EGFR, Ki67 and p16 (P > 0.05). It showed statistically significant correlation between expression of EGFR and the ability of erosion of middle ear cholesteatoma (P 0.05). The expression of EGFR, Ki67, p16 in all epithelial layers of middle ear cholesteatoma were abundantly stained, especially in the basal and spinous layers. Only the basal layer were slightly stained in control groups.@*CONCLUSION@#The expression of EGFR, Ki67, p16 in middle ear cholesteatoma was significantly high-er compared with the skin of external auditory of cholesteatoma patients and healthful peoples. There was correlation between the expression of EGFR or Ki67 and the ability of erosion of middle ear cholesteatoma. It means that EGFR, Ki67 and p16 play a key role in the hyperproliferation middle ear cholesteatoma.